Differential Pulse Voltammetric Determination of Clozapine in Drug Dosage Forms at Bismuth Film Electrode

Abstract

A new differential pulse voltammetric method for the determination of clozapine in drug dosage forms has been described. In this study, the amount of clozapine in commercial forms has been determined at bismuth modified glassy carbon electrode by taking advantage of the electrochemical oxidation of it. The glassy carbon electrode was modified with BiCl3 to prepare the bismuth film electrode. The acetate buffer solution at pH = 5.00 was selected as the supporting electrolyte in where the maximum current was observed for clozapine at the bismuth film electrode. The effect of pH on peak current and peak potential of clozapine was investigated by differential pulse voltammetry (DPV), and the effect of scan rate on the peak current was examined by cyclic voltammetry (CV). With this developed voltammetric method, the detection limit for clozapine in the working range of 1×10-6 M and 10×10-6 M was found to be 6.112×10-9. The amount of clozapine in the drug tablets was stated after the determination of analytical parameters. The recovery study was executed to check the accuracy and precision of the applied method.