From Microfluidic Paper-Based Analytical Devices to\nPaper-Based Biofluidics with Integrated Continuous Perfusion

Abstract

As\na kind of facile tool, microfluidic paper-based analytical devices\n(μPADs) have been widely used in analytical and biomedical fields.\nHowever, because we lack the ability to control the continuous perfusion\nof these devices, they are not generally used in fields that require\ncontinuous flow, especially biofluidics fields such as cell culturing,\ndrug screening, and organs on chips. In this study, we designed a\nnovel, low-cost, and compact platform that can be used to control\nthe continuous perfusion of μPADs. As most of the parts of this\nplatform can be created using a three-dimensional (3D) desktop printer,\nour platform can be easily duplicated by other researchers. We demonstrate\nthat with our system, μPADs can be promising paper-based biofluidic\nplatforms for cell culturing and drug screening.