Direct Electron Transfer of Cytochrome C on Cadmium Oxide Nanoparticles Modified Carbon Paste Electrode

Abstract

In this study, the direct electrochemistry of cytochrome c immobilized on a modified carbon paste electrode with cadmium oxide nanoparticles was described. The prepared cadmium oxide nanoparticles were characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). The direct electron transfer of the immobilized cytochrome c exhibited two couples of redox peaks with the formal potentials of -0.305 V and -0.244 V (vs. SCE) in 0.1M (pH 7.0) PBS, respectively. The biosensor displayed an excellent electro-catalytical response to the reduction of H2O2. The linear range of this biosensor for H2O2 determination was from 20 to 210μM. The established biosensor exhibited fast response, high sensitivity, good reproducibility and stability.