Direct Electron Transfer of Myoglobin on CdO Nanoparticles Modified Glassy Carbon Electrode

Abstract

In this study, direct electron transfer of myoglobin (Mb) on cadmium oxide (CdO) nanoparticles modified glassy carbon electrode (GCE) was investigated. Prepared CdO nanoparticles were studied by X-Ray diffraction (XRD) and UV–visible absorption methods. All electrochemical studies were performed by cyclic voltammetry (CV) and a potentiostat device. The cyclic voltammogram of Mb/ CdO Nps/ GCE showed a couple of stable redox and oxidative peaks at -470 and -370 mV at scan rate of 50 mVs−1, respectively. The formal potential (E°) of myoglobin was calculated as -(420 ± 3) mV. Direct electrode transfer led to design a biosensor for determination of hydrogen peroxide (H2O2). The sensor sensitivity was found in the range of 50 to 1150 μM of H2O2. Designed biosensor showed a good reproducibility and stability.