Electrochemical Determination of Cisplatin in Serum at Graphene Oxide/Multi-walled Carbon Nanotubes Modified Glassy Carbon Electrode

Abstract

Graphene oxide dispersed multi-walled carbon nanotubes composite modified glassy carbon electrode (GO-MWNTs/GCE) was prepared and the GO-MWNTs/GCE was employed for the electrochemical determination of anticancer drug cisplatin. A pair of well-defined redox peak of cisplatin was observed at the GO-MWNTs/GCE in 0.05 M KCl solution (pH 7.4) and electrode process is adsorption- controlled. The result indicates that the GO-MWNTs composite materials can effectively improve the sensibility and performance of the electrode for cisplatin. Based on the GO-MWNTs/GCE, a differential pulse voltammetry (DPV) method for the determination of cisplatin was proposed. The analytical performance of the method was further evaluated in the presence of human 5% serum. A good linear relationship was obtained within the concentration range from 1.30 μM to 26.0 μM in the presence of 5% serum, with the linear regression equation Ipa (qA)= 345947c+3.2421 (r=0.9990). The detection limit for cisplatin in the presence of 5% human serum can reach to 0.113 μM.