Accelerating spontaneous Raman spectroscopy and imaging (Conference Presentation)

Abstract

Raman spectroscopy provides a comprehensive molecular information of a biological sample without any invasive and destructive treatment prior to a measurement. However, the slow measurement speed limits imaging applications of Raman spectroscopy. I present acceleration of Raman spectroscopy and imaging by multiplexed spatial sampling. Multiline illumination Raman microscopy, scanning a sample with multiple line-shaped laser foci, improved the imaging speed by 4 orders of magnitude in comparison to conventional confocal Raman microscopy. I also present a selective-sampling approach for accelerating Raman spectroscopy and imaging. I discuss the potential new applications of accelerated Raman spectroscopy and imaging in life sciences.