Electrochemical Detection of Tributyrin using Prussian Blue Functionalized Glassy Carbon Electrode

Abstract

Electrochemical biosensor is a self-contained integrated device, which can provide specific quantitative or semiquantitative analytical information using a biological recognition element (biochemical receptor) which is retained in direct spatial contact with an electrochemical transduction element. In this work, surface functionalization is done on the glassy carbon electrode (GCE) as the electrochemical sensor for detection of tributyrin (TB) as fat content measurement tool. Tributyrin is a triglyceride present in butter and can be described as a liquid fat with an acrid taste. This paper described about the cyclic voltammetry (CV) measurements on the functionalized GCE with graphene oxide (GO) and Prussian Blue (PB) immobilized with lipase (denoted as GC/GO/PB/Lipase electrode) dipped into the phosphate buffer solution (PBS pH 7.4). The functionalized electrode then used to measure the current response of different concentration of tributyrin ranged from (10 mg/ml to 1000 mg/ml). The results shows that current GC/GO/PB/Lipase electrode response was proportional to the tributyrin concentration. The proposed GC/GO/PB/Lipase bioelectrode for TB analysis offers an efficient derivative in the field of bioelectronics devices.