La muerte sin llanto: violencia y vida cotidiana en Brasil

Abstract

An actinobacterium strain PLK6-54^T was isolated from Lankwai peat swamp forest soil collected from Yala province, Thailand. Strain PLK6-54^T exhibited morphological and chemotaxonomic properties described for the genus Streptomyces. It formed a spiral spore chain directly on aerial mycelium. Growth was observed between 20 and 40 °C and at pH 5-8. The maximum NaCl for growth was 2 % (w/v). ll-Diaminopimelic acid, arabinose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside were detected as the phospholipids. The major menaquinones were MK-10(H₂) and MK-9(H₆). The major cellular fatty acids were iso-C_{16 : 0}, anteiso-C_{15 : 0} and iso-C_{14 : 0}. 16S rRNA gene sequence data supported the assignment of strain PLK6-54^T to the genus Streptomyces and showed that Streptomyces rubidus NBRC 102073^T (99.0 %) was the closest relative. Moreover, the average nucleotide identity-blast (85.5 %) and digital DNA-DNA hybridization (30.7 %) values reported between strain PLK6-54^T and its closest neighbour were below the threshold values for delineation of a novel species. Strain PLK6-54^T could be distinguished from related validly described Streptomyces species by several phenotypic properties. The combination of genotypic and phenotypic data indicated that strain PLK6-54^T is representative of a novel species of the genus Streptomyces. The name Streptomyces acidipaludis sp. nov. is proposed for strain PLK6-54^T. The type strain is PLK6-54^T (=TBRC 11250^T=NBRC 114297^T).