Development And Validation Of Capecitabine Tablet (Pharmaceutical Dosage Form) By Using Rp-Hplc Method.

Abstract

A new precise accurate and reliable validated method for the determination of Capecitabine was developed by using reverse phase high performance liquid chromatography in pharmaceutical dosage forms. Spectrophotometer determination was carried out at an absorption maximum of 240nm by using methanol. The linearity was over the concentration range of 20-120 µg/ml with correlation coefficient 0.999. Chromatographic separation was carried out by using a mobile phase of methanol: Acetonitrile: water (80:20:80 V/V) on Waters 2487 dual absorbance column in an isocratic mode at a flow rate of 1.1 ml/min with UV detection at 240 nm. The developed methods were found to be precise and accurate for the estimation of Capecitabine in pharmaceutical dosage forms and could be used for routine analysis. Keywords: Capecitabine, RP-HPLC, Spectrophotometry, Waters 2487 dual absorbance detector, Nova pack 300 × 3.9mm 5μ as column, 240nm