RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF LAMIVUDINE TABLET DOSAGE FORM

Abstract

ABSTRACT An accurate and precise HPLC method was developed for the determination of Lamivudine. Separation of the drug was achieved on a reverse phase hypersil BDS C18, 100 X 4.6 mm, 5µ using a mobile phase consisting of Buffer and acetonitril in the ratio of 65:35v/v. The flow rate was 1mL/min and the detection wavelength was 270nm. The linearity was observed in the range of 25% to 150% with a correlation coefficient of not less than 0.998. The %RSD of Lamivudine for In precision was found to be 0.15, 0.20 and 0.20, 0.05 respectively. The recovery was found to be 98% and 102% for Lamivudine respectively which shows the accuracy of proposed method. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of Lamivudine tablet dosage forms. Key words: Lamivudine, Method development, RP- HPLC