Synthesis of [4′‐¹³C] pyridoxol

Abstract

Abstract 5‐Cyano‐3‐hydroxy‐2‐methylpyridine‐l‐oxide, after O‐methylation with dimethylsulfate, was reacted regiospecifically with sodium [ 13 C] cyanide to give [4′‐ 13 C] 4,5‐dicyano‐3‐hydroxy‐2‐methylpyridine. The latter compound was successively transformed to [4′‐ 13 C] pyridoxol and [4′‐ 13 C] pyridoxal phosphate.