Study of Nano Hydroxyapatite/ Poly(Lactic Acid)-Polycaprolactone Nanofiber Composite Scaffolds

Abstract

At present, tissue engineering scaffolds in the process of clinical medicine plays an irreplaceable important role.Based on the in-situ precipitation combined with frozen extraction, good n-HA dispersion and three-dimensional (3D) structure of n-HA/PLLA-PCL composite nanofibrous scaffolds were prepared.The effects of the proportion of polymer, gel temperature and the amount of calcium phosphate solution on the morphology of nanofibrous scaffolds were investigated.From the experimental results,it can be concluded that the scaffolds' morphology have changed while the proportions of the polymer are different, and with more PCL, the fiber diameter increases.At low gel temperature (-10°C or lower), n-HA/PLLA-PCL composite scaffolds could be able to get relatively uniform nano fiber network structure, while at high gel temperature (0°C or higher), nanofibers tend to flake growth, support structure is more compact.Also, with the adding of (NH 4 ) 2 HPO 4 solution, nanometer fiber diameter increases, however, the degree of uniformity decreases.