Amperometric Biosensor for Hydrogen Peroxide Determination Based on Black Goat Liver-Tissue and Ferrocene Mediation

Abstract

흑염소의 간-조직과 ferrocene을 탄소반죽 전극에 고정하여 과산화수소를 정량할 수 있는 전극을 만들었다. Ag/AgCl 전극에 대하여 $-0.3{\sim}+0.0\;V$의 전위 범위에서 전극의 감도를 관찰하였으며 전극의 감응시간은 12 s로 나타났다. 전극의 검출한계는 2.25${\times)10^{-6}M$ (S/N＝3)이었으며,1.0${\times}10^{-2 }$M 과산화수소를 이용해 15회 반복 측정한 결과, 상대표준편차는 1.87%이었다. 또한, 방해물질의 영향도 없는 것으로 나타났다. 효소전극의 감도는 19일 사용 후 50%로 감소하였다. The response characteristics of the bioelectrode developed by the co-immobilization of black goat liver tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide were evaluated. In the range of electrode potential examined ($-0.3{\sim}+0.0\;V$ vs. Ag/AgCl), the response time was relatively short (t95%=12 s) and it responded in the wide range of pH. The detection limit was 2.25${\times)10^{-6}M$ and a relative standard deviation of the measurements which were repeated 15 times using 1.0${\times}10^{-2 }$M hydrogen peroxide was 1.87%. The bioelectrode sensitivity decreased to 50% of the original value in 19 days of continuous use.