Calcium: Cytoplasmic, Mitochondria, Endoplasmic Reticulum, and Flux Measurements

Abstract

Fluorescent dyes that change their spectral properties in the presence of calcium are available. They have been used to measure the changes in calcium levels which occur in cells when stimulated by a variety of agonists. Methods for measuring receptor-mediated calcium flux and for tracking calcium movement within the cell and the movement of internal calcium stores are described.