牛痘ウイルスの増殖および組織培養家兎腎の相互作用に関する組織化学的並びに生化学的研究 第2篇 生化学的研究

Abstract

This paper deals with metabolic changes in the rabbit kidney tissues cultured with vaccinia virus, as biochemically examined. The following results were obtained. 1. During the course of the cultures, both nucleic acids in the tissues were observed to decrease rapidly regardless of the presence or the absence of the virus, however, when turnover rate of the nucleic acids was determined using inorganic radio-phosphate as tracer, the rate was far higher in the tissues cultured with the virus than those cultured without the virus. These results are considered to suggest that assimilation and dissimilation of nucleic acids is brought about in a rapid pace as compared with that in non-infected controls. 2. Almost no difference was observed in the rate of endogenous respiration and of anaerobic glycolysis, in cytochrome oxydase activity and in succinoxydase activity, between the tissues cultured with the virus and the control tissues. However, it was remained to be examined by what kind of energy-producing system the virus multiplies in the host cells in this case. 3. The increase in lactic acid was oberved to be more remarkable in the tissues cultured with the virus than in the control tissues, however, further investigation will be needed for determining what metabolic activity is responsible for the increase in lactic acid in the tissues.