Rapid Measurement\nof Residual Kanamycin Using Highly\nSpecific Biomimetic Recognition Paper-Based Chip

Abstract

The development of highly specific biomimetic recognition\nmaterial\nis a challenge for rapid detection of harmful residues in foodstuff.\nIn this study, a paper-based boronate affinity metal–organic\nframework/molecularly imprinted polymer microfluidic chip (FZS-BA@MIP)\nwas constructed based on the in situ construction strategy, which\nwas also designed as a highly specific biomimetic recognition module.\nHere, the homogeneous zeolitic imidazole framework-8 (ZIF-8) membrane\nserved as a great scaffold and enrichment layer. Besides, the recognition\nlayer of MIP was prepared based on a highly oriented boronate affinity\nsurface imprinting strategy. With the aid of the liquid flow channel,\nthe highly specific enrichment and visual detection for antibiotic\nresidues like kanamycin in actual products were achieved on the paper\nchip module of an integrated lateral flow platform. The whole analysis\nprocess could be accomplished within 30 min. In brief, this study\noffered a new integrated biomimetic recognition platform for visually\ndetecting harmful veterinary residues containing <i>cis</i>-diols, which demonstrated promising commercial value in point-of-care\ntesting of foodborne hazardous compounds.