MOESM1 of An engineered cryptic Hxt11 sugar transporter facilitates glucose–xylose co-consumption in Saccharomyces cerevisiae

Abstract

Additional file 1. Supplementary figures. Figure S1. Growth (○). glucose utilization (□) and ethanol production (●) by the control strain DS68616 expressing all endogenous Hxt transporters and containing the empty vector (A), and the transporter-deficient strain DS68625 expressing Hxt11 (B). Figure S2. Xylose uptake by strain DS68625 expressing HXT2 (●) or HXT11 (○) in the presence of increasing glucose concentrations (0-500 mM). Figure S3. Growth of strain DS68616 (■) and DS71054 (□) on 2 % glucose (A) or 2 % xylose (B). Figure S4. Maximum exponential growth rate of strain DS68625 expressing various Hxt11-N366X mutants on 2 % glucose (A) or 2 % xylose (B). The black bar indicated the wild-type position, and the gray bar indicates the N366D mutant obtained in the error-prone mutagenesis. The dashed line indicates the growth rate of strain DS68625 without any introduced transporter. The error bars are from two technical samples. Figure S5. Fluorescence images of strain DS68625 expressing GFP fusion proteins of Hxt11-N366X mutants grow on 2 % maltose. Figure S6. Consumption of xylose and glucose at 1.0 % each by the transporter-deficient strain DS68625 expressing Hxt11 (A) or Hxt11-N366T (B). Symbols: glucose (□), xylose (■), and ethanol (○). The error bars are from two technical samples. Figure S7. Ratio of the Xylose and glucose consumption rate in the presence of glucose. Strain DS68616 with the empty vector (●), or strain DS68625 with a vector expressing Hxt11(○), Hxt11 N366M (■) or Hxt11 N366T (□). Data calculated from the fermentation profiles presented in Fig. 3.