Additional file 1 of Kidney tissue regeneration using bioactive scaffolds incorporated with differentiating extracellular vesicles and intermediate mesoderm cells

Abstract

Additional file 1: Table S1: List of primer sequences used for RT-qPCR in vitro (for human genes) Table S2: List of primer sequences used for RT-qPCR in vivo (for mouse genes) Figure S1: (A) Nitric oxide release profile analyzed with NO sensor and (B) standard curve of NO current depending on concentration Figure S2: (A) Cell images of cellular morphological changes resulting from IM differentiation. (B) The gene expression levels of renal differentiation factors by different medium (APEL and CDM) by RT-qPCR (n = 3). Figure S3: The fluorescent images for (A) dEV and ?(B) IM incorporation into PMEZ scaffolds using confocal microscopy. Green: DiO-labeled dEVs, Blue: Hoechst-labeled IM (Scale bars equal to 40 µm) Figure S4: (A) The gene expression levels of LHX1 in IMctrl, IMdF, and IMdEV_2D by RT-qPCR (n = 3). (B) The gene expression levels of LHX1 in IMctrl, IMdF, and IMdEV_3D by RT-qPCR (n = 3). Figure S5: (A) The fluorescence-based immunohistochemistry of AQP-1 expression of scaffolds at 2 weeks after implantations (Scale bar: 100 µm). (B) The fluorescence-based immunohistochemistry of Nephrin expression of scaffold at 2 weeks after implantations (Scale bar: 100 µm). Figure S6: The gene expression levels of pro-inflammatory factors (TNF-α and IL-8) on the mouse model at (A) 2 and (B) 8 weeks after implantations (n = 3). Figure S7: The fluorescence-based immunohistochemistry of KIM-1 expression of scaffolds at 2 weeks after implantations (Scale bars equal to 100 µm) Figure S8: The development of renal differentiation