Additional file 1 of IRES-mediated Pichia pastoris cell-free protein synthesis

Abstract

Additional file 1: Fig. S1 Full PJL1-Crpv-Kozak-sfGFP sequence. Fig. S2 PCR products of 14 IRES analyzed by agarose gel electrophoresis. Fig. S3 Flow chart of the preparation of cell extracts. Fig. S4 Standard quantitative curve of sfGFP based on the fluorescence value and sfGFP protein expression level. Table S1 Information of strain used as cell extract. Table S2 Information of IRES used in this study. Table S3 Information of reagents.