Soybean Peroxidase-Catalyzed Oxidation of Luminol by\nHydrogen Peroxide

Abstract

Anionic soybean peroxidase <i>Glycine max</i> (SbP) is shown to efficiently catalyze luminol oxidation by\nhydrogen peroxide. Contrary to horseradish peroxidase, the presence of <i>p</i>-iodophenol in the reaction\nmedium affects slightly the efficiency of SbP catalysis. A maximal intensity of chemiluminescence,\nproduced through this enzymatic reaction, was detected at pH 8.4−8.6. Contrary to anionic palm\ntree peroxidase, in the presence of SbP, chemiluminescence intensity increases with the reaction\nbuffer concentration. The detection limit of SbP in the reaction of luminol oxidation is 0.3 × 10^-12 M.\nTherefore, high sensitivity in combination with the long-term chemiluminescent signal is indicative of\ngood prospects for application of this enzyme in enzyme immunoassay with chemiluminescent\ndetection.\nKeywords: Peroxidase; soy; chemiluminescence; luminol; hydrogen peroxide; enhancement