Self-Healing Hyaluronic\nAcid Nanocomposite Hydrogels\nwith Platelet-Rich Plasma Impregnated for Skin Regeneration

Abstract

The development of natural hydrogels with sufficient\nstrength and\nself-healing capacity to accelerate skin wound healing is still challenging.\nHerein, a hyaluronic acid nanocomposite hydrogel was developed based\non aldehyde-modified sodium hyaluronate (AHA), hydrazide-modified\nsodium hyaluronate (ADA), and aldehyde-modified cellulose nanocrystals\n(oxi-CNC). This hydrogel was formed <i>in situ</i> using\ndynamic acylhydrazone bonds <i>via</i> a double-barreled\nsyringe. This hydrogel exhibited improved strength and excellent self-healing\nability. Furthermore, platelet-rich plasma (PRP) can be loaded in\nthe hyaluronic acid nanocomposite hydrogels (ADAC) <i>via</i> imine bonds formed between amino groups on PRP (<i>e</i>.<i>g</i>., fibrinogen) and aldehyde groups on AHA or oxi-CNC\nto promote skin wound healing synergistically. As expected, ADAC hydrogel\ncould protect and release PRP sustainably. In animal experiments,\nADAC@PRP hydrogel significantly promoted full-thickness skin wound\nhealing through enhancing the formation of granulation tissue, facilitating\ncollagen deposition, and accelerating re-epithelialization and neovascularization.\nThis self-healing nanocomposite hydrogel with PRP loading appears\nto be a promising candidate for wound therapy.