Abietane Diterpenoids with Antioxidative Damage Activity\nfrom <i>Rosmarinus officinalis</i>

Abstract

Nine\nnew and nineteen known compounds were isolated and identified\nfrom Rosmarinus officinalis under the\nguidance of bioassay and LCMS. They all belonged to abietane diterpenoids\nwhich enriched the types of compounds in R. officinalis, especially the discovery of a series of 20-norabietane diterpenoids\n(<b>4</b>, <b>6</b>–<b>9</b>, and <b>26</b>–<b>27</b>). The antioxidative damage activity of the\ncompounds was tested on H₂O₂ damaged SH-SY5Y\ncells. Compounds <b>5</b>, <b>6</b>, and <b>7</b> presented moderate ability for promoting the growth of damaged cells.\nCompounds <b>10</b>, <b>11</b>, <b>13</b>–<b>20</b>, <b>27</b>, and <b>28</b> displayed a high\nantioxidative damage effect whose cell viability rates were more than\n80%. The antioxidative damage effect of <b>11</b>, <b>16</b>, <b>18</b>, and <b>20</b> were higher than that of EGCG\n(positive control) in which <b>11</b>, <b>18</b>, and <b>20</b> were the acetylated derivatives of carnosic acid (<b>10</b>), 7α-methoxy-isocarnosol (<b>16</b>), and carnosol\n(<b>19</b>), respectively. It suggested that 10-carboxyl/formyl\nof abietane diterpenoids was essential for maintaining the antioxidative\ndamage activity and the adjacent hydroxyl groups on the benzene ring\nwas less important for holding the bioactivity. These acetylated derivatives\nwith high bioactivity and stability could be regarded as new sources\nof antioxidants or antioxidative damage agents being used in the food\nand medical industry.