MOESM1 of Bacterial conversion of depolymerized Kraft lignin

Abstract

Additional file 1: Figure S1. (a) The SEC chromatogram of 1 g/L depolymerized (at 220 °C, 5 mL/min) lignin. Red dotted lines represent the fractions collected. The collected fraction at 95–108 min corresponds to the 0.2–0.4 kDa peak in the SEC chromatograms calibrated with PEG standards. (b) UHPLC chromatograms of the fractions obtained from SEC. The peaks in the fraction 95–108 min correspond to aromatic monomers (Vanillin-3.5 min; guaiacol-4.6 min; acetovanillone-4.7 min). Table S1. Results of homology BLAST with the previously well-characterized DyP proteins against the genome of the organisms used in this study. P. putida EM42 strain used in this study is the modified version of KT2440 and hence the genome of KT2440 (parental strain) was used for BLAST searches. Proteins with identity more than 75% are emphasized in green. Proteins that were found absent and the ones with less than 30% query are highlighted in red. P. fluorescens highlighted in blue is the only organism with proteins similar to DyPC and DyPD. Table S2. Results of homology BLAST with laccases against the genome of the organisms used in this study. P. putida EM42 strain used in this study is the modified version of KT2440 (parental strain) and hence the genome of KT2440 was used for BLAST searches. Proteins with identity more than 75% are emphasized in green. Table S3. Growth of bacterial strains on agar plates with 5 mM vanillin/guaiacol as the only source of carbon in M9 medium, incubated for 2 weeks at 30 °C. The isolates were previously tentatively reported as Pseudomonas sp. (B); P. plecoglossicida (C); P. deceptionensis (9.1); and Rhodococcus erythropolis (19). Duplicate experiments were performed and the growth results were similar (++ abundant growth; + growth; − no growth). R. opacus was the only organism to show growth on guaiacol (highlighted).