Adsorption of Berberine Hydrochloride, Ligustrazine\nHydrochloride, Colchicine, and Matrine Alkaloids on Macroporous Resins

Abstract

This research aims at identifying\nsuitable resin adsorbents for\nefficient separation and purification of alkaloids from plant materials.\nThe adsorption properties (equilibrium, kinetics, and column breakthrough)\nof four alkaloid model compounds (berberine hydrochloride, ligustrazine\nhydrochloride, colchicine, and matrine) on selected macroporous resins\nwere studied. The adsorption equilibrium capacities and desorption\nratios of the four model compounds on nine different macroporous resins\nwere measured and compared. It was observed that the resins with a\nlow polarity and high surface area offered a high adsorption capacity\nfor all alkaloids. The pseudo-second-order adsorption rate equation\nfit well all the kinetic data, and the Langmuir and Freundlich isotherm\nequations correlate well the adsorption isotherms on the four resins.\nAmong the nine resins studied in this work, the HPD300 resin was identified\nas the most promising adsorbent for alkaloids separation and purification\nbecause of its excellent adsorption and desorption properties for\nall four alkaloid compounds. The adsorption breakthrough experiment\non the HPD300 resin using a mixture solution containing all four model\ncompounds further confirmed the effective separation of alkaloids\non the HPD300 resin.