Promoting Diabetic Oral Mucosa Wound Healing With Saa Hydrogel

Abstract

Aim or purpose: In diabetic patients, compromised angiogenesis due to endothelial dysfunction leads to delayed intraoral wound healing. However, the moist and dynamic environment of the oral cavity impedes the use of normal wound dressings. Sulfated chitosan (SCS) is a promising biomaterial that promoting angiogenesis. Here, a light-responsive hydrogel combined with SCS explored intraoral wound healing. We designed a SCS-modified hydrogel combined with alginate Methacryloyl (AlgMA) and acrylamide (AM) and demonstrated efficient wet adhesion properties suitable for the wet and dynamic oral environment. Materials and methods: 1. Synthesis and Characterization of Hydrogels 2. Biocompatibility Evaluation 3. Cell Culture 4. Diabetic Rat Oral Mucosal Wound Healing Model Results: 1. SAA hydrogel was synthesised successfully; 2. SAA hydrogel demonstrated excellent wet-adhesive ability and retention time (72 hours) on rat palatal mucosa in vitro; 3. SAA hydrogel transitioned to gel state within 5 seconds under light exposure; 4. SAA Hydrogel exhibited favorable cell compatibility; 5. SAA hydrogel Promoted Diabetic Rat Oral Mucosa Defects Healing by promoting angiogenesis in vivo; 6. SAA Hydrogel promoted angiogenesis of high-glucose treated HUVECs; 7. SAA hydrogel can regulate immunity by shifting macrophage polarization from M1 to M2; 8. SAA hydrogel regulated the crosstalk between macrophages and HUVECs to accelerate diabetic oral mucosa defects healing. 9. SAA hydrogel enhanced VEGF secretion of macrophage treated with high-glocuse and promoting angiogenesis by VEGF-VEGFR2/PI3K-AKT pathway under high-glucose. Conclusions: This study found that SCS-loaded hydrogel accelerates diabetic oral wound healing via angiogenesis, enhanced by macrophage phenotype modulation.