PREPARATION AND IN VITRO WOUND HEALING EFFECTS OF ANIMAL-BASED PLATELET-RICH PLASMA LYSATE

Abstract

Wound healing is a vital field of study in both human and veterinary medicine, particularly in the treatment of chronic wounds. Platelet-rich plasma, known for its potential to enhance tissue regeneration due to its high concentration of growth factors, can be further processed into Platelet-rich plasma lysate. As an acellular product, Platelet-rich plasma lysate may exhibit reduced immunogenicity and can be stored frozen for future applications. Using heterologous Platelet-rich plasma lysate derived from larger animal species can be beneficial, particularly for veterinary patients who are unable to provide their own blood due to conditions such as low body weight, coagulopathy, or anemia. This study aims to evaluate and compare the in vitro wound-healing effects of Platelet-rich plasma lysate derived from equine, porcine, and caprine sources on dermal fibroblasts. Platelet-rich plasma lysate was prepared using a double centrifugation method and freeze-thaw protocol, and its effects were assessed through cell viability and in vitro wound healing assays. The cell viability assay revealed a positive dose-dependent effect of Platelet-rich plasma lysate from all tested animal species. Notably, 10% and 20% Platelet-rich plasma lysate from caprine and equine sources, as well as 20% caprine Platelet-rich plasma lysate, showed significantly higher cell viability compared to the negative control (p < 0.05). The in vitro wound healing assay showed that all Platelet-rich plasma lysate from the selected species had positive cell migration effects on dermal fibroblasts comparable to the positive control (10% Fetal Bovine Serum) at 5% concentration. However, a negative dose-dependent effect followed at higher concentrations. When compared to negative control, only positive control (10% Fetal Bovine Serum), 5% porcine Platelet-rich plasma lysate, as well as 5% and 10% caprine Platelet-rich plasma lysate were found to have significantly higher cell migration effect on dermal fibroblasts at 48-hour post-scratch. These findings highlight the potential of animal-derived Platelet-rich plasma lysate particularly of caprine origin, as an alternative treatment for wound healing, though further in vivo studies are needed to confirm its clinical applicability.