RECOMBINANT PRODUCTION OF THE P60 ANTIGEN OF LISTERIA MONOCYTOGENES

Abstract

Listeria monocytogenes is a significant foodborne pathogen characterized by an intracellular life cycle, pronounced adaptive mechanisms, and a broad spectrum of virulence factors. The aim of this study was to obtain the recombinant p60 antigen of L. monocytogenes from isolates circulating in the Republic of Kazakhstan. Using recombinant DNA technology, a producer strain Escherichia coli ArcticExpress(DE3)/Lmp60 was generated, enabling the expression of the protein with a molecular mass of 50.3 kDa. The specificity of the recombinant p60 antigen was confirmed by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. The obtained results highlight the diagnostic value of the p60 protein of L. monocytogenes as a target antigen for the development of highly specific ELISA-based test systems aimed at the rapid identification of L. monocytogenes in food products.