Evaluation of Anti-anxiety Activity of Ethanolic Extract of Doronicum Hookeri Rhizome in Swiss Albino mice

Abstract

The Aim of this study is the “Evaluation of anti-anxiety activity of Ethanolic Extract of plant Doronicum Hookeri Rhizome in Swiss Albino Mice.” Methods: Twenty Swiss Albino Mice are of either sex selected with weight 20-30gm. Elevated Plus Maze and Light and Dark Exploration test was performed on them with full safety and hygiene. This consists of a 10cm x 10cm central platform, elevated 50cm off the ground, connected to two open arms 50x10cm and two closed arms 50cm x 40cm x 10cm. Swiss albino rats weighing 20-40g were treated with OS extract, diazepam, and gum arabic for 30 min and then individually placed in the middle of an increasing number of arm diameters. Closing time is 5 minutes for both arms to open and close. Elapsed time is in seconds. The number of entries into the open and closed arms was counted during the test. Entry means that all four paws are on the arms, and in Light and Dark Exploration test: the installation consisted of two square boxes, each measuring 50cm x 50cm x 50cm, separated by wooden walls. One of the boxes is dark and the other is illuminated by a 7W/12V lamp. There is an opening (6cm x 6cm) in the middle of the wooden wall that can be opened and closed using a transparent Plexiglas sliding door through which the animals can move from both sides. Mice were placed individually in the center of the light box and monitored for the next 5 min Time spent in both boxes in seconds. The number of passes in the box is also indicated. Mice were treated with OS extract, diazepam, for 30 min being placed in the light box. Result: Ethanolic extract of Doronicum Hookeri is found satisfactory, results show the increase in time spent in Open Maze and decreased in closed maze. Similarly time spent in Open Arena is increased as in closed arena. The value is calculated as Controlled, Standard, Low Dose and High Dose. Conclusion: The ethanolic extract showed significant anti-anxiety effect (p<0.04) when compared to control group.