A fluorescence and colorimetric dual-mode LAMP method for detection of <i>Vibrio parahaemolyticus</i>

Jinling Cui; Yanling Wang; Chao Jiang; Lihao Wang; Lei Wang; Chao Shi; Cuiping Ma

doi:10.1039/d5ay00407a

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A fluorescence and colorimetric dual-mode LAMP method for detection of Vibrio parahaemolyticus

Jinling Cui Yanling Wang Chao Jiang Lihao Wang Lei Wang Chao Shi Cuiping Ma

Year: 2025 Journal: Analytical Methods Vol: 17 (19)Pages: 3925-3932 Publisher: Royal Society of Chemistry

DOI: 10.1039/d5ay00407a

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Abstract

A dual-mode LAMP method integrating fluorescence detection and visual colorimetric detection was proposed for detection of V. parahaemolyticus . The method has good specificity and can accurately detect 1 pg μL −1 of V. parahaemolyticus DNA.

Keywords:

Vibrio parahaemolyticus Fluorescence Dual mode Chromatography Chemistry Biology Optics Bacteria Physics Engineering Genetics

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Biosensors and Analytical Detection

Physical Sciences → Engineering → Biomedical Engineering

Vibrio bacteria research studies

Life Sciences → Biochemistry, Genetics and Molecular Biology → Endocrinology

Aquaculture disease management and microbiota

Life Sciences → Immunology and Microbiology → Immunology

A fluorescence and colorimetric dual-mode LAMP method for detection of Vibrio parahaemolyticus

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