Chronic Toxicity Investigations with Nilavembu Kudineer in Sprague-Dawley Rats

Abstract

The safety assessment of Nilavembu Kudineer (NVK), a polyherbal formulation with a wide spectrum of medicinal applications, was meticulously conducted in Sprague-Dawley rats. Over the course of 90 days, NVK was orally delivered at dosages of 100, 200, and 400 mg/kg to test for chronic toxicity. The results of this research provide important information on the relative safety of NVK and its possible effects on human health. Neither death nor any treatment-related adverse clinical manifestations were recorded during the chronic investigation. Feed intake and body weight increase were similar across animals given NVK, with the exception of a little decrease in females in the 200 and 400 mg/kg groups. No adverse findings were found in the ocular investigations performed as part of the safety study. Comprehensive analyses of urinalysis, hematological parameters, and biochemical markers subsequent to NVK administration identified minor alterations in select parameters at distinct dose levels, without any overarching systemic effects. Importantly, both gross and histopathological examinations revealed an absence of lesions directly attributable to NVK treatment. The findings contribute substantial insights to the realm of herbal medicine, providing a solid foundation for the responsible integration of NVK into diverse healthcare contexts.