Biogenic Selenium Nanoparticles Loaded Alginate-Gelatin Scaffolds for Potential Tissue Engineering Applications

Abstract

Selenium nanoparticles (SeNPs) were reported for its anticancer and antimicrobial properties. Alginate and gelatin scaffolds can act as an important biomaterial, more specifically in bone tissue engineering. Green synthesis of SeNPs from Luffa cylindrica (LC) and loading of SeNPS with alginate-gelatin scaffold and to check its biocompatibility. The SeNPs were prepared via the green synthesis method and loaded into an alginate-gelatin scaffold. Characterization studies such as UV-Vis spectroscopy, FTIR, and SEM were carried out in LC-SeNPs and Se-NPs loaded scaffold. The hydrophilicity of the scaffolds was determined using water contact angle measurements. Annexin V PI assay was conducted to determine the biocompatible nature of prepared SeNPs-loaded alginate-gelatin scaffolds. The UV-VIS spectrum gave an intense peak at 266 and 384 nm, whereas the FTIR gave a strong peak at 3500-500 cm-1 fingerprint regions. SEM images showed flower-shaped LC-SeNPs and their distribution of SeNPs on the surface of alginate-gelatin scaffolds. water contact angle measurement was found to be 29.21°. Cell viability results showed 78.11% viable cells following treatment with alg-gel-Se scaffold, revealing its biocompatibility towards peripheral blood mononuclear cells. Overall, it could be concluded that the SeNP-loaded alg-gel scaffold is a promising candidate for tissue engineering, but further studies are required to confirm its potential role. Keywords- Selenium nanoparticles, Alginate-gelatin scaffolds, tissue engineering, biocompatibility.