High-Throughput Single-Cell Sorting by Stimulated Raman Activated Cell Ejection

Jing Zhang; Haonan Lin; Jiabao Xu; Meng Zhang; Xiaowe Ge; Wei E. Huang; Ji‐Xin Cheng

doi:10.1364/fio.2023.fm6e.5

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JOURNAL ARTICLE

High-Throughput Single-Cell Sorting by Stimulated Raman Activated Cell Ejection

Jing Zhang Haonan Lin Jiabao Xu Meng Zhang Xiaowe Ge Wei E. Huang Ji‐Xin Cheng

Year: 2023 Pages: FM6E.5-FM6E.5

DOI: 10.1364/fio.2023.fm6e.5

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Abstract

We report high-throughput single-cell sorting by stimulated Raman-activated cell ejection. For 1 μm polymer beads mixture, we achieved yield ~95%, purity ~98%, and throughput ~14 eps. Bacteria and live fungi were also tested.

Keywords:

Throughput Sorting Raman spectroscopy Cell sorting Cell Materials science Chemistry Computer science Optics Biochemistry Telecommunications Physics Algorithm

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Microfluidic and Bio-sensing Technologies

Physical Sciences → Engineering → Biomedical Engineering

3D Printing in Biomedical Research

Physical Sciences → Engineering → Biomedical Engineering

Spectroscopy Techniques in Biomedical and Chemical Research

Life Sciences → Biochemistry, Genetics and Molecular Biology → Biophysics

High-Throughput Single-Cell Sorting by Stimulated Raman Activated Cell Ejection

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