Antibacterial activity of ethanolic extract from Derris scandens against human pathogenic bacteria

Abstract

The purpose of this study was to assess the antibacterial activity of an ethanolic extract of Derris scandens against clinically isolated human pathogenic bacteria. Materials and methods: The ethanolic extract was assessed for antibacterial activity against ten human pathogenic bacteria at different concentrations (25 - 100 μg/ml) through the agar well diffusion method. Group 1 (25μg/ml), Group 2 (50μg/ml), Group 3 (75μg/ml) Group 4 (100μg/ml), Group 5 (positive control), and Group 6 (negative control) and calculated the zone of inhibition. Results and discussion: The ethanolic extract showed antibacterial activity against 8 clinical bacterial strains of the 10 pathogens tested. The highest concentration (100μg/ml) of the ethanolic extract showed a maximum of 20 and 22mm inhibition zone against E. coli and S. typhi . The mean values were 0.130, 0.141, 0.117, 0.194, and 0.120 The sample size was calculated with a pretest G power of 80%. The sample size per group is 6, and the total sample size is 60. Conclusion: The effective bacterial inhibition rate of Derris scandens might provide a promising beneficial agent against bacterial infection and help to develop future infectious disease drugs.