Electrochemical Behavior of Sudan II and Its Determination in Food Using Activated Glassy Carbon Electrode

Abstract

A glassy carbon electrode activated in sulfuric acid was applied in the determination of Sudan II in food samples. The experimental parameters, such as supporting electrolyte, scanning rate and accumulation time, were tested. It was very clear that the redox peak currents of Sudan II obviously increased. In pH 4.0 citric acid-phosphate buffer solution, the oxidation peak current of Sudan II at the activated glassy carbon electrode was linearly proportional to its concentration in a range from 4.00 x 10-7 mol L-1 to 1.00x 10-5 mol L-1. The limit of detection was estimated by gradually decreasing the concentration levels of Sudan II and the detection limit was found to be 8.00 x 10-8 mol L-1. The electrochemical parameters was Calculated and mechanism related to the electrode reaction of Sudan II was proposed. The sensor showed great promise for the simple, sensitive, and quantitative detection of Sudan II and was used for the determination of Sudan II in real food samples with satisfactory results.