Simultaneous Determination of Adenine and Thymine in Presence of Guanine at Electrochemically Activated Glassy Carbon Electrode

Abstract

Here we report simple cyclic voltammetry method for electrochemical activation of Glassy carbon electrode (GCE) by 0.1M sodium bicarbonate (NaHCO3) solution without acidic medium. Under optimized condition, the electrochemically activated glassy carbon electrode (EAGCE) was applied for individual and simultaneous determination of DNA bases adenine (Ade) and Thymine (Thy) in presence of high concentration of Guanine (Gu) for the first time. The surface study was examined using scanning electron microscope (SEM). Linear sweep voltammetry (LSV) method shows the oxidation of Ade, Thy and Gu individually. Moreover the simultaneous determination of Ade and Thy in presence of high concentration of Gu also exhibited using LSV. The EAGCE detects in the range of 2 μM to 230 μM for Ade, 100 μM to 2.3 mM for Thy and 10 μM to 0.2 mM for Gu respectively. The low limit of detection was found as 0.1 μM, 0.11 μM and 1.01 μM for Ade, Thy and Gu respectively. Additionally the proposed sensor exhibits good repeatability, reproducibility and sufficient stability.