Amperometric Detection of Glucose by Polyaniline-Activated Carbon Composite Carbon Paste Electrode

Abstract

In this study, a novel carbon paste electrode using the salt form of polyaniline (pani)-activated carbon composite sensitive to glucose, was prepared. Glucose oxidase enzyme was immobilized to modified carbon paste electrode by cross-linking with glutaraldehyde. Determination of glucose was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V vs. Ag/AgCl. The effects of pH and temperature were investigated and optimum parameters were found to be 5.0 and 65 °C, respectively. The linear working range of the electrode was 5.0×10-7 - 1.0×10-5 M, R2 =0.980. The storage stability and operation stability of the enzyme electrode were also studied.