Electrodeposition of Nickel Nanoparticles Modified Glassy Carbon Electrode for Nonenzymatic Glucose Biosensing

Abstract

We report the electrocatalytic activity of nickel nanoparticles (NPs) modified glassy carbon electrode (GCE), prepared by potentiostatic electrodeposition, toward glucose electro-oxidation in alkaline medium. The morphology image of nickel on the GCE surface was characterized by scanning electron microscope (SEM). Electrochemical oxidation of glucose with this sensor was examined by cyclic voltammetry (CV) and chronoamperometry in alkaline aqueous solutions. Amperometric reponse of the electrocatalytic oxidation to glucose at the potential of 500 mV presents a linear dependence (R=0.9941) in the glucose concentration range of 5 μM-1.155 mM, with a 1 μM detection limit (S/N = 3). The biologic compounds probably existed in human serum sample, such as ascorbic acid, uric acid, and dopamine, do not disturb the determination of glucose. The relative standard deviation (RSD) of the determination of practical serum samples is less than 7% compared with the results obtained from clinical examination. The proposed sensor exhibits excellent electrocatalytic activity toward the oxidation of glucose, which is a good candidate for glucose quantification.