Short Communication: Amperometric Immunosensor of Procalcitonin Based on Amplification Strategy of Ferrocene-Modified Gold nanoparticles

Abstract

In this article a novel electrochemical immunoassay for procalcitonin (PCT) detection based on ferrocene-modified Au nanoparticles was developed and ferrocene-modified gold nanoparticles provided the electrochemical amplification signal; the nanoparticles was labeled with PCT antibodies which could bind with PCT and another PCT-antibody-modified gold electrode to form a sandwich structure. The PCT immunosensor exhibited good linearity of oxidization current when the potential is 0.3 V and PCT concentration lies between 1.5 pg/mL and 50 ng/mL, and the limit of detection was 0.8 pg/mL. Moreover, the PCT immunosensor was successfully applied to detect PCT in clinical samples.