Milk Casein Coated Iron Oxide Nanoparticles As Superparamagnetic Core/Shell Carrier for Biomedical Areas

Abstract

In this research we have characterized features of super paramagnetic iron oxide nanoparticles (SPIONs) as multifunctional nanoparticles for use in biomedical fields. Several techniques measurements such as DLS, zeta potential, FT-IR and VSM analysis were used to characterization the size and special features of naked SPIONs and TCT modified SPIONs. DLS results indicated the size distributions of aqueous TCT-SPIONs were found to be relatively narrow. FT-IR and VSM results appointed that TCT-SPIONs utilize functional covalent binding on the surface of nanoparticles. After that the SPIONs are coated with casein and indicate that there is an interaction between them. Moreover, we have investigated magnetic properties and anticancer effects of casein coated SPIONs. Therefore we showed casein could be used to increase the biocompatibility of the surface of SPIONs. At the end we show that bonding of Bis(indolyl)methane (BIM) to the surface of casein coated SPIONs have good magnetite properties for targeted drug delivery. Cell culture study was carried out in MCF-7 and PC-3 cell lines. The cell culture studies showed BIM-core/shell SPIONs provide good anticancer effects against both breast and prostate cancer cell lines. The results advised these nanoparticles as good drug carrier system.