Assessment of phytochemicals and antioxidant activity of custard apple fruit pulp (Annona squamosa Linn.)

Abstract

Annona squamosa Linn. (Custard apple) has been used drastically in India traditional remedy for treatment of dysentery, cardiac problems, fainting, worm infections, constipation, hemorrhage, dysuria, fever. This study assessed the phytochemicals present in the fruit pulp of A. squamosa. The phytochemical was extracted one by one with distilled water and 96% ethanol. A wide variety of pharmacologically energetic compounds such as alkaloids, coumarins, tannins, cardiac glycosides, flavonoids, carbohydrates, phenols, and saponins were found to present in the fruit pulp of A. squamosa. However, terpenoids and phlobatannins had been absent in this plant. This study additionally assessed the contents of phenolics and flavonoids for their in vitro antioxidant activity. The complete polyphenol content material of ethanol extract of A. squamosa measured with the aid of Folin-Ciocalteau reagent in terms of gallic acid equal completed 238.68±4.13 mg GAE/g. The flavonoid content material of the plant pattern as quercetin equivalent executed 84,75±0.82 mg QE/g. The antioxidant undertaking of the ethanol extract of A. squamosa was correlated with complete phenolic and flavonoid content material with values IC50 of 134.761±1.83 µg/ml, 62.63±0.43 µg/ml for DPPH and ABTS scavenging activity, respectively.