Evaluation of Antimicrobial Cream Containing Chromolaena odorata Methanolic Leaves Extract for Wound Healing

Abstract

Abstract: Plants are an essential source of potentially beneficial compounds for developing new medicinal drugs. Chromolaena odorata have been reported to have antimicrobial properties because they contain secondary metabolites flavonoid, terpenoid, tannin, terpenoid, alkaloid and phenolic compound. These compound affects antimicrobial activity and have the ability in the wound healing process. Therefore, this study was carried out to formulate and evaluate the antimicrobial cream from C. odorata methanolic extract. The leaves of C. odorata were collected, prepared, and extracted using a standard procedure with 95% methanol. Six formulations from two bases were used to develop an antimicrobial cream from C. odorata methanolic extract. The physical characteristics were evaluated on all cream formulations, such as organoleptic test, homogeneity, creaming properties, centrifugation and thermal cycle test. Determination of pH and antimicrobial activity assessment was observed after 24 hours, one week, one month, two months and three months of storage. All formulated creams were observed with dark green colour, homogenous and fragrant leaves smell. Their appearance was semi-solid, with F1 to F3 being very soft and oily while F4 to F6 being very soft and fatty. Formulation of F4, F5, and F6 was stable in the physicochemical and organoleptic tests. The pH formulated creams were between 4 to 5. Within three months of storage, the result that demonstrated no significant differences were F1 and F3 in pH measurement, F2, F3, and F5 in antibacterial activity, and F1, F3, and F5 in antifungal activity evaluation. In conclusion, out of the six formulations, the F3 formulation had the best stability during storage, antimicrobial activity, and minimal changes throughout the study. Thus, the F3 formulation is suitable as an antimicrobial cream for wound healing treatment. Keywords: Chromolaena odorata, antimicrobial cream, cream formulation, stability. Title: Evaluation of Antimicrobial Cream Containing Chromolaena odorata Methanolic Leaves Extract for Wound Healing Author: Norlizawati Ishak, Norhanizan Usaizan International Journal of Life Sciences Research ISSN 2348-313X (Print), ISSN 2348-3148 (online) Vol. 11, Issue 1, January 2023 - March 2023 Page No: 55-64 Research Publish Journals Website: www.researchpublish.com Published Date: 08-March-2023 DOI: https://doi.org/10.5281/zenodo.7708778 Paper Download Link (Source) https://www.researchpublish.com/papers/evaluation-of-antimicrobial-cream-containing-chromolaena-odorata-methanolic-leaves-extract-for-wound-healing