Amniotic membrane/silk fibroin-alginate nanofibrous scaffolds containing Cu-based metal organic framework for wound dressing

Abstract

Due to the skin's critical role in protecting the body from external injury, substantial research has been performed to improve abnormalities, such as cuts or burns. Today, many wound dressings are manufactured in various forms, each with distinct features. Due to their biocompatibility and biodegradability, biopolymer nanofibers like Silk Fibroin and Sodium Alginate are viable options for wound dressings. In the present study, a hybrid scaffold with Sodium Alginate and Silk Fibroin biopolymers was fabricated via hybrid electrospinning on a decellularized human Amniotic membrane (hAM) to treat chronic wounds. A Copper-based metal-organic framework was employed to protect the wound area against Escherichia coli and Staphylococcus aureus (two common micro-organisms available in the wound area), which was evaluated using the dilution method (colony count), and a reduction in the presence of bacteria was detected. Additionally, Mesenchymal stem cell viability was seen due to the addition of Cu-MOF. Furthermore, hAM boosted the adhesion and proliferation of Mesenchymal Stem Cells in prepared scaffolds and increased water wettability, which is critical during the wound healing process. These findings indicate that nanofibers containing Cu-based MOFs are promising materials for biomedical applications.