JOURNAL ARTICLE
Cas12a‐based on‐site, rapid detection of genetically modified crops
Zhiqiang DuanXiaoliang YangXingkun JiYing ChenXiaomu NiuAnping GuoJian‐Kang ZhuFeng LiZhaobo LangHui Zhao
Year: 2022 Journal: Journal of Integrative Plant Biology Vol: 64 (10)Pages: 1856-1859 Publisher: Wiley
Abstract
A CRISPR/LbCas12a-based nucleic acid detection method that uses crude leaf extracts as samples and is rapid (≤40 min for a full run) and highly sensitive (0.01%) can be used to monitor genetically modified organisms in the field.
Keywords:
CRISPR Loop-mediated isothermal amplification Computational biology Nucleic acid Genetically modified organism Genetically engineered Trans-activating crRNA Table (database) Biology Computer science Molecular biology Biological system Database Genetics Genome editing DNA Gene
Metrics
19
Cited By
2.34
FWCI (Field Weighted Citation Impact)
9
Refs
0.84
Citation Normalized Percentile
Is in top 1%
Is in top 10%
Citation History
Topics
CRISPR and Genetic Engineering
Life Sciences → Biochemistry, Genetics and Molecular Biology → Molecular Biology
Advanced biosensing and bioanalysis techniques
Life Sciences → Biochemistry, Genetics and Molecular Biology → Molecular Biology
Innovation and Socioeconomic Development
Social Sciences → Business, Management and Accounting → Business and International Management
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