Preparation and characterization of PEGylated capric acid liposomes for intravenous delivery system

Abstract

Colloid Laboratory, Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), MARDI Headquarters, Persiaran MARDI-UPM, 43000 Serdang, Selangor, Malaysia E-mail: [email protected], [email protected] Manuscript received online 16 August 2019, revised 20 August 2019, accepted 05 December 2019 Fatty acid liposomes showed a promising alternative as a carrier for various active ingredients to distribute intravenously. However, upon storage, fatty acid liposomes are unstable due to oxidation and aggregation. In this study, capric acid has been explored as a functional material for the preparation of liposomes. Fourier-transform infrared spectra, thermal activity, and phase transition of capric acid have been evaluated from the present experiment. Capric acid was spontaneously formed liposomes at pH 5.4 to 7.8. Incorporation of the fatty acid anchored poly ethylene glycol, namely 1,2-dioleoyl-sn-glycerol-3- phosphoethanolamide-N-[methoxy (poly ethylene glycol)-2000] (DOPEPEG2000) reduced the average particle size of liposomes to 110.8 nm and –8.5 mV at the molar ratio of capric acid to DOPEPEG2000 of 1:0.020. PEGylated capric acid liposomes showed a significantly stable upon storage as compared to bare capric acid liposomes. Physical characterization showed that PEGylated capric acid liposomes have the characteristics that may compatible with intravenous delivery.