Sensitive and Homogeneous Detection Systemwith Aptamer-based Biosensor

Abstract

Rapid and highly sensitive methods for detecting target molecules are required to develop biomarker-based diagnoses that help medication or therapy.Thus, the development of homogeneous sensing methods with high sensitivity can provide the basis for detecting a biomarker in routine diagnostics and on site.Oligonucleotide aptamers, ligands that consist of nucleic acids, have been selected for numerous targets such as proteins and small compounds, and are applicable to biosensing platforms.In particular, attractive features of aptamers enable us to construct valuable sensing methods for clinical uses without the need to use nanomaterials.Recent discoveries of novel signal transducing elements derived from an enzymatic and a fluorescent oligonucleotide also allow for the development of homogeneous aptasensors.In this review, we describe recent progress in the development of aptamer-based biosensors with high sensitivity.