A Facile Electrochemical Fabrication of Reduced Graphene Oxide-Modified Glassy Carbon Electrode for Simultaneous Detection of Dopamine, Ascorbic Acid, and Uric Acid

Abstract

This paper describes the simple fabrication of an electrode modified with electrochemically reduced graphene oxide (ERGO) for the simultaneous electrocatalytic detection of dopamine (DA), ascorbic acid (AA), and uric acid (UA). ERGO was formed on a glassy carbon (GC) electrode by the reduction of graphene oxide (GO) using linear sweep voltammetry. The ERGO/GC electrode was formed by subjecting a GO solution (1 mg mL-1 in 0.25 M NaCl) to a linear scan from 0 V to −1.4 V at a scan rate of 20 mV s-1. The ERGO/GC electrode was characterized by Raman spectroscopy, Fourier transform infrared spectroscopy, contact angle measurements, electrochemical impedance spectroscopy, and cyclic voltammetry. The electrochemical performance of the ERGO/GC electrode with respect to the detection of DA, AA, and UA in 0.1M PBS (pH 7.4) was investigated by differential pulse voltammetry (DPV) and amperometry. The ERGO/GC electrode exhibited three well-separated voltammetric peaks and increased oxidation currents during the DPV measurements, thus allowing for the simultaneous and individual detection of DA, AA, and UA. The detection limits for DA, AA, and UA were found to be 0.46, 77, and 0.31 μM respectively, using the amperometric i-t curve technique, with the S/N ratio being 3. Keywords: Dopamine, Graphene, Reduced graphene oxide, Electrochemical sensor