Poly(vinyl alcohol)-based Electrospun Nanofibers: Characterization and Phytase Immobilization

Ümran Duru Kamacı; Ayşegül Peksel; M Morshed; N Behary; N Bouazizi; J Guan; V Nierstrasz; L Ellis; N Rorrer; K Sullivan; M Otto; J Mcgeehan; Y Roman-Leshkov; N Wierckx; G Beckham; A Kumari; R Rajeev; L Benny; Y Sudhakar; A Varghese; G Hegde; A Naramittanakul; S Buttranon; A Petchsuk; P Chaiyen; N Weeranoppananti; X Lei; J Weaver; M Azain; S Gharibzahedi; B Smith; E Mullaney; A Ullah; K Bhavsar; J Khire; S Hussain; S Hanif; A Sharif; F Bashir; H Iqbal; T Coutinho; P Tardioli; C Farinas; U Rathnayake; T Senapathi; C Sandaruwan; S Gunawardene; V Karunaratne; N Kottegoda; Z Aytac; S Dogan; T Tekinay; T Uyar; J Santos; E Zavareze; A Dias; Vanier; B Joshi; E Samuel; Y Kim; A Yarin; M Swihart; S Yoon; C Isik; G Arabaci; Y Dogac; I Deveci; M Teke; V Godakanda; H Li; L Alquezar; L Zhao; L Zhu; R Silva; G Williams; Y Zhang; C Zhang; Y Sun; S Cheng; W Lu; Y Wang; P Zhang; Q Yao; D Sharma; S Saha; B Satapathy; U Guzik; K Hupert-Kocurek; D Wojcieszyska; A Peksel; H Azak; O Secgin; O Suer; F Aydin; V Turkoglu; Z Bas; A Allafchian; S Kalani; P Golkar; H Mohammadi; S Jalali; S Soni; S Sarkar; P Selvakannan; D Sarkar; S Bhargava; A Manjunath; M Irfan; K Anushree; K Vinutha; N Yamunarani; U Duru Kamaci; T Zhao; X Yong; Z Zhao; V Dolce; Y Li; R Curcio; Y Dersjant-Li; A Awati; H Schulze; G Partridge; B Bonine; P Polizelli; G Rodriguez; M Naghshbandia; H Moghimia; B Latif; H Yildiz; Caliskan; M Kamaci; A Caliska; H Yilmaz; J Harati; S Siadat; H Taghavian; S Kaboli; S Khorshidi; N Dutta; D Raj; N Biswas; M Mallick; S Omesh

doi:10.33263/briac126.75737583

JOURNAL ARTICLE

Poly(vinyl alcohol)-based Electrospun Nanofibers: Characterization and Phytase Immobilization

Ümran Duru Kamacı Ayşegül Peksel M Morshed N Behary N Bouazizi J Guan V Nierstrasz L Ellis N Rorrer K Sullivan M Otto J Mcgeehan Y Roman-Leshkov N Wierckx G Beckham A Kumari R Rajeev L Benny Y Sudhakar A Varghese G Hegde A Naramittanakul S Buttranon A Petchsuk P Chaiyen N Weeranoppananti X Lei J Weaver E Mullaney A Ullah M Azain S Gharibzahedi B Smith E Mullaney A Ullah K Bhavsar J Khire S Hussain S Hanif A Sharif F Bashir H Iqbal T Coutinho P Tardioli C Farinas U Rathnayake T Senapathi C Sandaruwan S Gunawardene V Karunaratne N Kottegoda Z Aytac S Dogan T Tekinay T Uyar J Santos E Zavareze A Dias Vanier B Joshi E Samuel Y Kim A Yarin M Swihart S Yoon C Isik G Arabaci Y Dogac I Deveci M Teke V Godakanda H Li L Alquezar L Zhao L Zhu R Silva G Williams Y Zhang C Zhang Y Wang Y Sun S Cheng W Lu Y Wang P Zhang Q Yao D Sharma S Saha B Satapathy U Guzik K Hupert-Kocurek D Wojcieszyska U Duru Kamaci A Peksel U Duru Kamaci A Peksel H Yildiz M Kamaci H Azak O Secgin O Suer F Aydin V Turkoglu Z Bas A Allafchian S Kalani P Golkar H Mohammadi S Jalali S Soni S Sarkar P Selvakannan D Sarkar S Bhargava A Manjunath M Irfan K Anushree K Vinutha N Yamunarani U Duru Kamaci M Kamaci T Zhao X Yong Z Zhao V Dolce Y Li R Curcio Y Dersjant-Li A Awati H Schulze G Partridge B Bonine P Polizelli G Rodriguez M Naghshbandia H Moghimia B Latif H Yildiz Caliskan M Kamaci A Caliska H Yilmaz J Harati S Siadat H Taghavian S Kaboli S Khorshidi N Dutta D Raj N Biswas M Mallick S Omesh

Year: 2021 Journal: Biointerface Research in Applied Chemistry Vol: 12 (6)Pages: 7573-7583

DOI: 10.33263/briac126.75737583

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Abstract

The aim of this study is to immobilization phytase obtained from cowpea seeds into nanofiber-based on poly(vinyl alcohol) (PVA) and to investigate kinetic properties, optimal pH, and temperature of free and immobilized phytase. The structural analysis and morphological properties of the nanofibers are carried out via SEM and XRD. The results indicated that enzyme stability, pH, and thermal stability are increased after immobilizing phytase into the nanofiber. The optimum pH and temperature of the free and immobilized phytase are found as pH 5.0 and 45-65 oC, respectively. These results indicated that the immobilized phytase could be a good candidate for agriculture, animal feed, food, and medical applications.

Keywords:

Vinyl alcohol Phytase Nanofiber Thermal stability Immobilized enzyme Electrospinning Chemistry Nuclear chemistry Chemical engineering Materials science Polymer chemistry Enzyme Organic chemistry Polymer Composite material

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Electrospun Nanofibers in Biomedical Applications

Physical Sciences → Materials Science → Biomaterials

Phytase and its Applications

Life Sciences → Agricultural and Biological Sciences → Plant Science

Cassava research and cyanide

Life Sciences → Agricultural and Biological Sciences → Plant Science

Poly(vinyl alcohol)-based Electrospun Nanofibers: Characterization and Phytase Immobilization

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