LOW CONCENTRATION PHENOL DETECTION USING PSEUDOMONAS FLUORESCENS BIOFILM IMMOBILIZED ON SCREEN PRINTED CARBON ELECTRODE (SPCE)

Abstract

A new method of phenol measurement based on cyclic voltammetry has been tried using Pseudomonas fluorescens biofilm as a bioreceptor. The research is aimed at developing a new measurement for detecting phenol and related compounds using bacterial biofilm. Therefore, it can be further developed for in situ phenol and related compounds monitoring. The trial evaluated several variables, such as optimum pH value during measurement and biofilm development time. Measurement was conducted at a relatively low concentration (1 ppb – 1 ppm). Based on the obtained data, the optimum measurement was at pH 8 and on the 6th day of developing bacterial biofilm that produced the maximal electrical current. The analytical determination revealed that the sensitivity was at 0.0433 mA/ppm with an R2 value of 0.9208. The apparent limit of detection value was extrapolated around 0.1 ppm; thus, it is suggested that biofilm had potency for measuring phenol at low concentrations. However, this trial needs improvements on biofilm stabilization for broader measured phenol concentrations.