Optical-sectioning super-resolution structured illumination microscopy for fast virtual pathology

Abstract

On-site pathology of the surface of resected tumors provides real-time assessments of surgical procedure and improve treatment outcomes. Tissue preparation is a major bottleneck of standard pathology procedures that can be overcome by virtual pathology imaging. Here, we use optical sectioning super-resolution structured illumination microscopy (OS-SR-SIM) with a low magnification objective. Low magnification objective provides a higher field of view, and super-resolution reconstruction retrieves lost resolution due to lower NA. We successfully resolved sub-resolution fluorescent beads in highly scattering media that were not resolvable with standard OS-SIM with an NA 0.45 objective. Human tissue testing is underway.