lncRNA UASR1 is overexpressed in oral squamous cell carcinoma and regulates cancer cell proliferation by regulating miR‑375/JAK2 axis

Abstract

Long non-coding RNAs (lncRNAs) have been confirmed to participate in cancer regulation, including oral squamous cell carcinoma (OSCC). The aim of the present study was to investigate the role of UASR1 in OSCC. The expression levels of UASR1, miR-375 and JAK2 were detected in OSCC tissues by reverse transcriptase quantitative PCR. The targets of UASR1 were predicted by IntaRNA. Colony formation and CCK-8 assays were conducted to estimate cell proliferation. Western blotting was used to detect the protein expression of JAK2. The results demonstrated that UASR1 was upregulated in OSCC tissues compared with non-tumor tissues, and the high level of UASR1 expression was associated with poor overall survival. UASR1 is predicted to interact with miR-375 and the interaction was confirmed by Dual-luciferase activity assay. However, overexpression of UASR1 and miR-375 did not affect the expression of each other. Instead, upregulation of JAK2, a target of miR-375, was observed after the overexpression of UASR1 in OSCC cells. Moreover, overexpression of UASR1 attenuated the inhibitory effects of miR-375 on the expression of JAK2 and cell proliferation. Therefore, UASR1 is overexpressed in OSCC and regulates cancer cell proliferation by regulating the miR-375/JAK2 axis.